Facilities

The research activities are performed in the building of the G. Ronzoni Foundation.  The Institute has about 1,000 sq. meters of laboratory and office space, including chemistry, biochemistry (including a walk-in cold room) and instrumental laboratories, fully equipped with state-of-the-art instrumentation, dedicated to chemical preparations, biochemistry and structural research. Research facilities include the LC-MS, chromatography, physico-chemical laboratories and NMR Centre 

  • High-resolution 600 MHz Bruker Avance III 600
    • Quadruple resonance (H/C/N/2H) High-sensitivity CryoProbe TCI 5mm
  • High-resolution 500 MHz Bruker NEOn500
    • Quadruple resonance (H/C/N/2H) High-sensitivity CryoProbe TCI 5mm
    • Triple resonance probe TXI 5mm
    • Inverse detection multinuclear NB probe 5mm with a shielded x-axix gradient BGU gradient unit Z (50 gauss/cm)
  • High-resolution 500 MHz Bruker Avance HD
    • Inverse detection multinuclear BB probe 10mm
    • TXI 5mm probe
    • solid state probe – MASSB-DR-BB/1H&19F-3.2mm

  • UHR-QqTOF (Ultra-High Resolution Qq-Time-Of-Flight), Impact II Bruker Daltonics, (UHPLC Platin Blue Knauer)
  • ESI-Q-TOF MS, micrOTOFQ Bruker Daltonics (HPLC Dionex Ultimate 3000)
  • ESI-FT MS, Solarix high mass accuracy and resolution, Bruker Daltonics (HPLC Agilent 1100)

 

  • Multidetector GPC Sec Systems
    • 2 Viscotek units equipped with Triple Detector Array (RI, viscometer and RALLS/MALLS) and related software for absolute Mw determinations.
    • Omnisec Multidetector system (Malvern Instruments)
  • Viscosimeter: Anton Paar MCR92
  • DLS/Z Potential:
    • Viscotek 802 DLS Dinamic Lighit Scattering
    • Malvern Instr. Zetasizer Nano ZS

 

  • Centrifuge
  • UV
  • Fluorimeter
  • Electrophoresis
  • Size-exclusion and ion-exchange chromatography
  • Ultrafiltration
Understanding how glycosaminoglycans become substrates of the Heparanase enzyme suggest new idea or strategies to inhibit this enzyme
Software
  • NAMD 12.0, VMD 1.9.3, Ambertools 14.0, Gamess (US), Autodock 4.2, CORCEMA, NOEPROM,
  • R, RStudio (R foundation for statistical computing, Vienna)
Hardware
  • Selected MD simulations were run on remote supercomputers at CINECA SCAI (Super Computing Applications and Innovation). The applied supercomputers were Fermi, Marconi, Galileo Clusters www.hpc.cineca.it
  • Workstation (E4 Engineering) double CPU (Intel(R) Xeon(R) Gold 6138, 20 core 2.00GHz) Memory (RAM) 96Gb, two HD 1Tb
  • Workstation (Dell Precision T7600) double CPU (Xeon 6Core 2.3 GHz), GPU NVIDIA Tesla C2075, total Memory (RAM) 32Gb, two HD 1Tb.
  • Workstation (Dell Precision T7500) CPU (Xeon 6Core 2.66 GHz), total Memory (RAM) 24Gb, 1 HD 1Tb.
  • Workstation (Acer) CPU (i7 3.4GHz, 8 Core); total memory (RAM) 8Gb, 1HD 1Tb.
  • Workstation (Mac mini) CPU (Intel Coreì7 2GHz), total memory (RAM) 8Gb, 2HD 500Gb, Operating System: OS X El Capitan.